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Four-compartmental kinetic model of the simultaneous processes occurring throughout biodegradation of polychlorinated biphenyls in the active bacterial cell suspension
Vrana, Branislav, Dercová, Katarína, Baláž, Štefan and Tandlich, Roman Four-compartmental kinetic model of the simultaneous processes occurring throughout biodegradation of polychlorinated biphenyls in the active bacterial cell suspension Acta Chimica Slovaca, Vol.1, No. 1, 2008, 329-349
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
acs_0029.pdf
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acs_0029.pdf |
application/pdf |
178.29KB |
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| Author(s) |
Vrana, Branislav
Dercová, Katarína
Baláž, Štefan
Tandlich, Roman
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| Title |
Four-compartmental kinetic model of the simultaneous processes occurring throughout biodegradation of polychlorinated biphenyls in the active bacterial cell suspension
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| Journal name |
Acta Chimica Slovaca
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
1
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| Issue number |
1
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| ISBN |
978-80-227-2957-4
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| Start page |
329
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| End page |
349
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| Total pages |
21
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| Place of publication |
Bratislava
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| Publisher |
Slovak Technical University
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| Collection year |
2008
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| Language |
english
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| Subject |
290000 Engineering and Technology
290100 Industrial Biotechnology and Food Sciences
270000 Biological Sciences
270100 Biochemistry and Cell Biology
270300 Microbiology
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| Abstract/Summary |
Kinetics of distribution of polychlorinated biphenyls (PCBs) in an active bacterial suspension of Pseudomonas stutzeri was studied by monitoring the evaporated amounts and the concentration remaining in the liquid medium containing the biomass of the degrader bacterium. A model considering biosorption, evaporation, and primary biodegradation of individual PCB congeners was constructed using the methods of subcellular toxicokinetics. The time hierarchy of the underlying processes (uptake/release of the congeners to/from biomass is much faster than evaporation and degradation) allowed for construction of a simplified disposition function, i.e. the time dependence of the intra- and extracellular PCB concentrations. The resulting model was fitted to the experimental data, with the parameters characterising biosorption and evaporation determined in separate experiments. In this way, the biodegradation rate constants were determined for individual congeners of the technical PCB mixture DELOR 103. Biodegradability decreases with increasing number of chlorines in the molecule, especially if they are located in the ortho- and para- positions. On the other hand, the increasing number of free 2,3-positions or their equivalents on the biphenyl skeleton leads to an increased biodegradability. A quantitative structure-degradability relationship with these variables was formulated for 2- and 3-chlorinated congeners.
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| Keyword(s) |
biosorption
biodegradation
evaporation
polychlorinated biphenyls
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