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Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials
Jakób, Alina, Bryjak, Jolanta and Polakovič, Milan Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials Chemical Papers, Vol.63, No. 6, 2009, 677-682
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Jakób, Alina
Bryjak, Jolanta
Polakovič, Milan
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| Title |
Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
6
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| ISSN |
0366-6352
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| Start page |
677
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| End page |
682
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
290000 Engineering and Technology
290100 Industrial Biotechnology and Food Sciences
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| Abstract/Summary |
The goal of the study was to find the most accurate and sensitive method for the determination of activity of pectinolytic enzymes in complex mixtures obtained from fruit materials such as raw cloudberry and raspberry juices. Several assay methods based on enzymatic reactions using viscometric, colourimetric, spectrophotometric, or pH-titration detection of the reaction products were tested. Problems with the application of the selected methods, such as very low detection signal or very large background signal, were observed. Among the tested methods, only a modified method based on the continuous recording of the released carboxyl groups titration allowed to assay the activity of exogenous pectin methylesterase with a good linearity, sensitivity, accuracy, and minimised the interference of other fruit components.
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