A rapid in vitro assay of cobalamin in human urine and medical tablets using ICP-MS

Zhang, Gai, Liu, Jianbo, Meng, Zuchao and Wang, Xin A rapid in vitro assay of cobalamin in human urine and medical tablets using ICP-MS Chemical Papers, Vol.63, No. 3, 2009, 366-370

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  

Author(s) Zhang, Gai
Liu, Jianbo
Meng, Zuchao
Wang, Xin
Title A rapid in vitro assay of cobalamin in human urine and medical tablets using ICP-MS
Journal name Chemical Papers
Publication date 2009
Year available 2009
Volume number 63
Issue number 3
ISSN 0366-6352
Start page 366
End page 370
Place of publication Poland
Publisher Versita
Collection year 2009
Language english
Subject 270000 Biological Sciences
270100 Biochemistry and Cell Biology
Abstract/Summary A rapid and simple as well as sensitive inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of cobalamin is described. Cobalamin in human urine and medicine tablet solutions was converted on-line into free cobalt ions in acid medium, the cobalt ions were then detected by ICP-MS. Cobalamin was determined by measuring the increase of integral counts per second intensity, which was linear over the cobalamin concentration range of 1.0 × 10−10 g mL−1 to 8.0 × 10−5 g mL−1, and the limit of detection was 0.05 ng mL−1 (3σ). At the pump rate of 30 rotations per minute, one analysis cycle of cobalamin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations of less than 5 %. The proposed procedure was applied successfully in monitoring cobalamin in human urine without any pretreatment process and in rapid determination of cobalamin in multivitamin tablets.
 
Related Links
Link Description
http://www.springerlink.com/content/513705g52643411t/?p=14783a1a790945f9bcc50...   fulltext  
 
 
User Comments
 
Access Statistics: 3 Abstract Views Detailed Statistics
Created: Mon, 22 Mar 2010, 07:46:41 CET by Jana Taptičová . Detailed History