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Influence of reaction medium composition on enzymatic synthesis of galactooligosaccharides and lactulose from lactose concentrates prepared from whey permeate
Adamczak, Marek, Charubin, Danuta and Bednarski, Włlodzimierz Influence of reaction medium composition on enzymatic synthesis of galactooligosaccharides and lactulose from lactose concentrates prepared from whey permeate Chemical Papers, Vol.63, No. 2, 2009, 111-116
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Adamczak, Marek
Charubin, Danuta
Bednarski, Włlodzimierz
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| Title |
Influence of reaction medium composition on enzymatic synthesis of galactooligosaccharides and lactulose from lactose concentrates prepared from whey permeate
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
2
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| ISSN |
0366-6352
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| Start page |
111
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| End page |
116
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
290000 Engineering and Technology
290100 Industrial Biotechnology and Food Sciences
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| Abstract/Summary |
The paper presents the results of investigations into the technological possibilities of controlling the transgalactosylation process of lactose in permeate after whey ultrafiltration in order to improve the efficiency of galactooligosaccharides or lactulose synthesis. The synthesis efficiency was influenced by the selection of a β-galactosidase preparation, substrate concentration and, in the synthesis of lactulose, also by the ratio of lactose and fructose added to the reaction mixture. The obtained synthesis efficiency of GOS and, most of all, of lactulose (65 g L−1), may be found satisfactory. The study also resulted in a proposed GOS or lactulose concentrates (concentrated or dried) production technology using permeate after ultrafiltration of milk or whey as lactose sources.
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