Rhizopus stolonifer LAU 07: a novel source of fructosyltransferase

Lateef, Agbaje, Oloke, Julius K., Kana, Evariste B. Gueguim, Oyeniyi, Solomon O., Onifade,Olukemi R., Oyeleye, Ayokunmi O. and Oladosu, Olabiyi C. Rhizopus stolonifer LAU 07: a novel source of fructosyltransferase Chemical Papers, Vol.62, No. 6, 2008, 635-638

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  

Author(s) Lateef, Agbaje
Oloke, Julius K.
Kana, Evariste B. Gueguim
Oyeniyi, Solomon O.
Onifade,Olukemi R.
Oyeleye, Ayokunmi O.
Oladosu, Olabiyi C.
Title Rhizopus stolonifer LAU 07: a novel source of fructosyltransferase
Journal name Chemical Papers
Publication date 2008
Year available 2008
Volume number 62
Issue number 6
ISSN 0366-6352
Start page 635
End page 638
Place of publication Poland
Publisher Versita
Collection year 2008
Language english
Subject 270000 Biological Sciences
270100 Biochemistry and Cell Biology
Abstract/Summary Increasing awareness of the importance of fructooligosaccharides (FOS) as ingredients of functional foods has led to intensive search of new sources of fructosyltransferases (FTase), enzymes responsible for the conversion of sucrose to fructooligosaccharides. A local strain of Rhizopus stolonifer isolated from spoilt orange fruit with high fructosyltransferase activity (U t) of 12.31–45.70 U mL−1 during a fermentation period of 24–120 h is herein reported. It showed low hydrolytic activity (U h) in the range of 0.86–1.78 U mL−1 during the same period. FOS yield of 34 % (1-kestose, GF2, nystose, GF3) was produced by FTase obtained from a 72 h-old culture using 60 g of sucrose per 100 mL of the substrate. When the isolate was grown in a defined submerged medium, its pH dropped sharply from the intial value of 5.5 to 1.0 within 24 h, and this value was maintained throughout the fermentation. The biomass content ranged from 8.8 g L−1 at 24 h of fermentation to reach the maximum of 10 g L−1 at 72 h. It was reduced to 5.6 g L−1 at the end of 120 h of fermentation. This report represents the first reference to a strain of Rhizopus as a source of FTase for the production of FOS. The high U t/U h ratio shown by this isolate indicates that it may be a good strain for the industrial and commercial production of FOS. However, there is a need of further optimization of the bioprocess to increase the conversion efficiency of sucrose to FOS by the enzyme.
 
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