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Computer-aided process design of affinity membrane adsorbers: a case study on antibodies capturing
Beijeren, Peter van, Kreis, Peter, Hoffmann, Achim, Mutter, Martina, Sommerfeld, Sven, Bäcker, Werner and Górak, Andrzej Computer-aided process design of affinity membrane adsorbers: a case study on antibodies capturing Chemical Papers, Vol.62, No. 5, 2008, 458-463
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Beijeren, Peter van
Kreis, Peter
Hoffmann, Achim
Mutter, Martina
Sommerfeld, Sven
Bäcker, Werner
Górak, Andrzej
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| Title |
Computer-aided process design of affinity membrane adsorbers: a case study on antibodies capturing
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| Journal name |
Chemical Papers
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
62
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| Issue number |
5
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| ISSN |
0366-6352
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| Start page |
458
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| End page |
463
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2008
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| Language |
english
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| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
Design of affinity membrane adsorbers for the purification of biomolecules requires a consideration of loading, washing, and elution. Modelling and simulation of membrane adsorbers in literature is, however, strongly focused on the loading step. Therefore, in this work, a complete process model which takes all the different steps into account was developed. Breakthrough experiments in which human IgG was captured onto and eluted from Sartobind Protein A downscale modules were used for model validation and for estimation of the required model parameters. The experimentally observed breakthrough curves were independent of the applied flow rate and from these results linear correlations between lumped kinetic parameters and linear velocity were determined. During elution, desorption was best described by an irreversible reaction of first order in H+ concentration. Applicability of the developed model to computer-aided design was illustrated through a process analysis study in which the influence of the amount of loaded protein per cycle on the process yield and productivity was investigated.
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