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Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1
Rymowicz, Waldemar, Rywińska, Anita and Gładkowski, Witold Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1 Chemical Papers, Vol.62, No. 3, 2008, 239-246
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Rymowicz, Waldemar
Rywińska, Anita
Gładkowski, Witold
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| Title |
Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1
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| Journal name |
Chemical Papers
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
62
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| Issue number |
3
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| ISSN |
0366-6352
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| Start page |
239
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| End page |
246
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2008
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| Language |
english
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| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
This study shows a possible microbial process for utilization of crude glycerol generated by the biodiesel industry for citric acid and erythritol production. Simultaneous production of citric acid and erythritol under nitrogen-limited conditions with glycerol as the carbon source was achieved with an acetate negative mutant of Y. lipolytica Wratislavia K1 in fed-batch cultivations. The effect of the initial glycerol concentration (from 30–180 g dm−3) on the citrate and erythritol production was investigated. As a result of the experiments, maximum citric acid production (110 g dm−3) and a very high amount of erythritol (81 g dm−3) were determined after 168 h of fed-batch cultivation with the initial glycerol concentration of 150 g dm−3 and the total glycerol concentration of 250 g dm−3. In addition, the citric acid to isocitric acid ratio of the products from this strain was 35.5:1.
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