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Influence of two-phase system composition on biocatalytic properties of β -galactosidase preparations
Bednarski, W. and Kulikowska, A. Influence of two-phase system composition on biocatalytic properties of β -galactosidase preparations Chemical Papers, Vol.61, No. 5, 2007, 364-369
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Bednarski, W.
Kulikowska, A.
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| Title |
Influence of two-phase system composition on biocatalytic properties of β -galactosidase preparations
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| Journal name |
Chemical Papers
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| Publication date |
2007
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| Year available |
2007
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| Volume number |
61
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| Issue number |
5
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| ISSN |
0366-6352
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| Start page |
364
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| End page |
369
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2007
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| Language |
english
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| Subject |
290000 Engineering and Technology
290100 Industrial Biotechnology and Food Sciences
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| Abstract/Summary |
A comparison was made between the catalytic properties of β-galactosidase from Penicillium canescens and Aspergillus oryzae using lactose in two-phase systems of an organic solvent and water. It was shown that the ability of β-galactosidase preparations to synthesize galactooligosaccharides depends on the type of the solvent used (cyclohexane, hexane, toluene) and on its proportion in the two-phase system. The use of cyclohexane in the reaction medium stabilized the activity of β-galactosidase from P. canescens which was immobilized on tosylated cotton cloth, and enabled its multiple use in galactooligosaccharides synthesis.
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