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Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier
Onderková, Z., Bryjak, J. and Polakovič, M. Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier Chemical Papers, Vol.61, No. 5, 2007, 359-363
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Onderková, Z.
Bryjak, J.
Polakovič, M.
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| Title |
Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier
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| Journal name |
Chemical Papers
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| Publication date |
2007
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| Year available |
2007
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| Volume number |
61
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| Issue number |
5
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| ISSN |
0366-6352
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| Start page |
359
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| End page |
363
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2007
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| Language |
english
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| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
Optimization of immobilization conditions was carried out for covalent binding of Aureobasidium pullulans fructosyltransferase to a copolymer of butyl acrylate and ethylene glycol dimethacrylate using a glutaraldehyde method. It was found that the highest activity of the preparation could be obtained for the immobilization pH 6.0 and initial protein amount 8.5 g per dm3 of the carrier. Effects of the reaction pH, temperature, and initial sucrose concentration on the activity and stability of the preparation were analyzed. Further investigations involved storage stability and operational stability in a mechanically stirred-tank reactor.
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