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Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system
Shao, X. D., Liu, H. Y., Gao, X. F., Chen, W. Q. and Song, Z. H. Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system Chemical Papers, Vol.61, No. 5, 2007, 353-358
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Shao, X. D.
Liu, H. Y.
Gao, X. F.
Chen, W. Q.
Song, Z. H.
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| Title |
Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system
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| Journal name |
Chemical Papers
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| Publication date |
2007
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| Year available |
2007
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| Volume number |
61
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| Issue number |
5
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| ISSN |
0366-6352
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| Start page |
353
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| End page |
358
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2007
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| Language |
english
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| Subject |
250000 Chemical Sciences
250400 Analytical Chemistry
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| Abstract/Summary |
A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.
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