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Optimization of ammonium nitrate concentration in single-stage continuous cultures of Aspergillus niger with biomass retention
Janczar, M. and Pietkiewicz, J. J. Optimization of ammonium nitrate concentration in single-stage continuous cultures of Aspergillus niger with biomass retention Chemical Papers, Vol.61, No. 3, 2007, 176-180
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Janczar, M.
Pietkiewicz, J. J.
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| Title |
Optimization of ammonium nitrate concentration in single-stage continuous cultures of Aspergillus niger with biomass retention
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| Journal name |
Chemical Papers
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| Publication date |
2007
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| Year available |
2007
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| Volume number |
61
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| Issue number |
3
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| ISSN |
0366-6352
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| Start page |
176
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| End page |
180
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2007
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| Language |
english
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| Subject |
290000 Engineering and Technology
290100 Industrial Biotechnology and Food Sciences
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| Abstract/Summary |
The effect of ammonium nitrate concentration in the citric acid biosynthesis by Aspergillus niger NC-12 in single-stage continuous cultures with biomass retention was investigated. Experiments were carried out in a BIOMER laboratory fermenter with 5 dm3 working volume. At the initial stage of each cultivation, the substrate in the bioreactor contained 1.5 g NH4NO3 dm−3. After 120 h onwards, the bioreactor was fed continuously at a constant dilution rate of 0.009 h−1. NH4NO3 concentration in the feed was varied from one culture to another, ranging between 0.5 g dm−3 and 2.5 g dm−3. Promising results were obtained when NH4NO3 concentration of 1.5 g dm−3 was used. The observed concentration of citric acid (c P) and yield of citric acid with respect to the introduced sucrose (Y P/S) were 117.88 g dm−3 and 78.59 %, respectively. The efficiency coefficient of citric acid biosynthesis (K ef) was very high, amounting to 83.38.
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