Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues

Ďurfinová, M., Brechtlová, M., Líška, B. and Barošková, Ž. Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues Chemical Papers, Vol.61, No. 4, 2007, 321-325

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  

Author(s) Ďurfinová, M.
Brechtlová, M.
Líška, B.
Barošková, Ž.
Title Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues
Journal name Chemical Papers
Publication date 2007
Year available 2007
Volume number 61
Issue number 4
ISSN 0366-6352
Start page 321
End page 325
Place of publication Poland
Publisher Versita
Collection year 2007
Language english
Subject 270000 Biological Sciences
270100 Biochemistry and Cell Biology
Abstract/Summary Two methods for determination of lipid peroxidation (LPX) products in rat brain homogenates were compared. The thiobarbituric acid (TBA) test and HPLC assay for analysis of malondialdehyde (MDA) were applied. Rat brain homogenate dissolved in tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) was mixed with TBA and H3PO4 and heated at 100°C to form colored complex that was extracted into butanol. No significant differences were found between the contents of TBA-reacting substances and their amount deduced from the MDA-TBA analysis. The presented results show that LPX products in brain homogenates can be determined without interferences also by the TBA test. Moreover, a survey of various methods used for the sample preparation before analysis of LPX products originating from different brain areas was made and compared with the obtained results.
 
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