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Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans
Platková, Z., Polakovič, M., Štefuca, V., Vandáková, M. and Antošová, M. Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans Chemical Papers, Vol.60, No. 6, 2006, 469-472
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Platková, Z.
Polakovič, M.
Štefuca, V.
Vandáková, M.
Antošová, M.
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| Title |
Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans
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| Journal name |
Chemical Papers
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| Publication date |
2006
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| Year available |
2006
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| Volume number |
60
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| Issue number |
6
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| ISSN |
0366-6352
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| Start page |
469
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| End page |
472
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2006
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| Language |
english
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| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
The study deals with the development of an immobilized biocatalyst for the production of fructooligosaccharides. Several commercial anion-exchange resins and polymethacrylate carriers were tested for the immobilization either by the direct attachment of fructosyltransferase or by the attachment accompanied by simultaneous glutaraldehyde crosslinking in the pH range from 5.7 to 7.1. On the basis of the efficiency of immobilization and the storage stability, Amberlite IRA 900 and Dowex Marathon MSA were selected as the best carriers for fructosyltransferase immobilization by direct attachment at pH 5.7.
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