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Dynamics of Evening Primrose Protein Hydrolysis
Golabczak, J., Strakowska, J. and Konstantynowicz, A. Dynamics of Evening Primrose Protein Hydrolysis Chemical Papers, Vol.59, No. 6a, 2005, 409-412
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
n596aa409.pdf
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596aa409.pdf |
application/pdf |
186.79KB |
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| Author(s) |
Golabczak, J.
Strakowska, J.
Konstantynowicz, A.
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| Title |
Dynamics of Evening Primrose Protein Hydrolysis
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| Journal name |
Chemical Papers
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| Publication date |
2005
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| Year available |
2005
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| Volume number |
59
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| Issue number |
6a
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| ISSN |
0366-6352
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| Start page |
409
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| End page |
412
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2005
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| Language |
english
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| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
Evening primrose protein isolate prepared by proteolysis was digested with trypsin at pH 8 and 50oC. Effects of enzyme and substrate concentrations and of digestion time on the degree of protein hydrolysis were determined. Experimental results were used to develop a mathematical model describing the path of enzymatic cleavage of evening primrose protein. According to this model, the process starts with the peptide bond hydrolysis followed either by formation of a stable enzymesubstrate complex or the peptide bond resynthesis, at the constant initial enzyme to substrate concentration ratio (ρ(E)0/ρ(S)0) and evening primrose protein concentrations of 20 to 50 g dm−3. Optimum proteolysis conditions (ρ(S)0 = 20 g dm−3, ρ(E)0/ρ(S)0 = 2 %, 5 h) provided the highest degree of protein hydrolysis of 28—30 %. Enzymatic digestion enhanced the content of low relative molar mass of peptides (easily absorbable in small intestine) in evening primrose protein hydrolyzate and increased its solubility in water.
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