An Enzymatic Sensor for the Analysis of Glycerol in Beverages

Lapenaite, I., Kurtinaitiene, B., Marcinkeviciene, L., Bachmatova, I., Laurinavicius, V. and Ramanavicius, A. An Enzymatic Sensor for the Analysis of Glycerol in Beverages Chemical Papers, Vol.55, No. 6, 2001, 345-349

Document type: ńĆl√°nok z ńćasopisu / Journal Article
Collection: Chemical papers  

Author(s) Lapenaite, I.
Kurtinaitiene, B.
Marcinkeviciene, L.
Bachmatova, I.
Laurinavicius, V.
Ramanavicius, A.
Title An Enzymatic Sensor for the Analysis of Glycerol in Beverages
Journal name Chemical Papers
Publication date 2001
Year available 2001
Volume number 55
Issue number 6
ISSN 0366-6352
Start page 345
End page 349
Place of publication Poland
Publisher Versita
Collection year 2001
Language english
Subject 250000 Chemical Sciences
250400 Analytical Chemistry
Abstract/Summary A biosensor for the determination of glycerol based on bacterial membranes was developed. Bacterial membranes containing pyrroloquinoline quinone (PQQ) dependent glycerol dehydrogenase (GlycDH) were isolated from Gluconobacter sp. 33 and were immobilized on the carbon rod electrode. Phenazine methosulfate (PMS) was applied as redox mediator. The response of the biosensor is not sensitive to the oxygen concentration in the bulk. Maximal selectivity of the biosensor was observed at pH 8. The calibration graph of the constructed biosensor was linear up to 8|10 mmol dm‚ąí3 glycerol and possessed the correlation coecient of 0.997. The selectivity of this biosensor to various compounds mostly found in the beverages was investigated. The concentration of glycerol in beverages was determined after biochemical elimination of glucose from the sample.
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