 |
An Enzymatic Sensor for the Analysis of Glycerol in Beverages
Lapenaite, I., Kurtinaitiene, B., Marcinkeviciene, L., Bachmatova, I., Laurinavicius, V. and Ramanavicius, A. An Enzymatic Sensor for the Analysis of Glycerol in Beverages Chemical Papers, Vol.55, No. 6, 2001, 345-349
|
|
|
Document type:
|
Článok z časopisu / Journal Article |
|
Collection:
|
Chemical papers
|
|
| Author(s) |
Lapenaite, I.
Kurtinaitiene, B.
Marcinkeviciene, L.
Bachmatova, I.
Laurinavicius, V.
Ramanavicius, A.
|
| Title |
An Enzymatic Sensor for the Analysis of Glycerol in Beverages
|
| Journal name |
Chemical Papers
|
| Publication date |
2001
|
| Year available |
2001
|
| Volume number |
55
|
| Issue number |
6
|
| ISSN |
0366-6352
|
| Start page |
345
|
| End page |
349
|
| Place of publication |
Poland
|
| Publisher |
Versita
|
| Collection year |
2001
|
| Language |
english
|
| Subject |
250000 Chemical Sciences
250400 Analytical Chemistry
|
| Abstract/Summary |
A biosensor for the determination of glycerol based on bacterial membranes was developed. Bacterial membranes containing pyrroloquinoline quinone (PQQ) dependent glycerol dehydrogenase (GlycDH) were isolated from Gluconobacter sp. 33 and were immobilized on the carbon rod electrode. Phenazine methosulfate (PMS) was applied as redox mediator. The response of the biosensor is not sensitive to the oxygen concentration in the bulk. Maximal selectivity of the biosensor was observed at pH 8. The calibration graph of the constructed biosensor was linear up to 8|10 mmol dm−3 glycerol and possessed the correlation coecient of 0.997. The selectivity of this biosensor to various compounds mostly found in the beverages was investigated. The concentration of glycerol in beverages was determined after biochemical elimination of glucose from the sample.
|
|
|
