 |
Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194
Antošová, M., Polakovič, M., Slovinská, M., Madlová, A., Illeová, V. and Báleš, V. Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194 Chemical Papers, Vol.56, No. 6, 2002, 394-399
|
|
|
Document type:
|
Článok z časopisu / Journal Article |
|
Collection:
|
Chemical papers
|
| |
| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
n566a394.pdf
|
566a394.pdf |
application/pdf |
217.36KB |
0 |
| Author(s) |
Antošová, M.
Polakovič, M.
Slovinská, M.
Madlová, A.
Illeová, V.
Báleš, V.
|
| Title |
Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194
|
| Journal name |
Chemical Papers
|
| Publication date |
2002
|
| Year available |
2002
|
| Volume number |
56
|
| Issue number |
6
|
| ISSN |
0366-6352
|
| Start page |
394
|
| End page |
399
|
| Place of publication |
Poland
|
| Publisher |
Versita
|
| Collection year |
2002
|
| Language |
english
|
| Subject |
270000 Biological Sciences
270100 Biochemistry and Cell Biology
|
| Abstract/Summary |
The e ect of the initial sucrose concentration (50 g dm−3, 200 g dm−3, and 350 g dm−3) and time of cultivation (1 d, 2 d, and 4 d) on the fructosyltransferase production by Aureobasidium pullulans CCY 27-1-1194 in shaken flask cultivations was investigated. It was found that the overall fructosyltransferase production depended strongly on the initial sucrose concentration when the highest value of enzyme activity (130 000 U dm−3) was achieved at 350 g dm−3 in the fourth cultivation day. The maximum of speci c activity per dry cell mass was achieved at the same sucrose concentration, but already in the second day. Since the second cultivation day, the ratio of the enzyme activity contained in cells and released into medium was 1.3—1.6, independently of sucrose concentration. In order to discuss the fructosyltransferase production in relation to cell growth and state of the culture, the sucrose conversion, produced amount of fructooligosaccharides and organic acids, and the yield factor of biomass on substrate, were determined as well.
|
|
|
|
