Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194

Antošová, M., Polakovič, M., Slovinská, M., Madlová, A., Illeová, V. and Báleš, V. Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194 Chemical Papers, Vol.56, No. 6, 2002, 394-399

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  
 
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Author(s) Antošová, M.
Polakovič, M.
Slovinská, M.
Madlová, A.
Illeová, V.
Báleš, V.
Title Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194
Journal name Chemical Papers
Publication date 2002
Year available 2002
Volume number 56
Issue number 6
ISSN 0366-6352
Start page 394
End page 399
Place of publication Poland
Publisher Versita
Collection year 2002
Language english
Subject 270000 Biological Sciences
270100 Biochemistry and Cell Biology
Abstract/Summary The e ect of the initial sucrose concentration (50 g dm−3, 200 g dm−3, and 350 g dm−3) and time of cultivation (1 d, 2 d, and 4 d) on the fructosyltransferase production by Aureobasidium pullulans CCY 27-1-1194 in shaken flask cultivations was investigated. It was found that the overall fructosyltransferase production depended strongly on the initial sucrose concentration when the highest value of enzyme activity (130 000 U dm−3) was achieved at 350 g dm−3 in the fourth cultivation day. The maximum of speci c activity per dry cell mass was achieved at the same sucrose concentration, but already in the second day. Since the second cultivation day, the ratio of the enzyme activity contained in cells and released into medium was 1.3—1.6, independently of sucrose concentration. In order to discuss the fructosyltransferase production in relation to cell growth and state of the culture, the sucrose conversion, produced amount of fructooligosaccharides and organic acids, and the yield factor of biomass on substrate, were determined as well.
 
 
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