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Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants
Šimková, Darina, Beinrohr, Ernest and Labuda, Ján Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants Acta Chimica Slovaca, Vol.2, No. 2, 2009, 129-138
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
acs_0052.pdf
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acs_0052.pdf |
application/pdf |
87.54KB |
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| Author(s) |
Šimková, Darina
Beinrohr, Ernest
Labuda, Ján
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| Title |
Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants
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| Journal name |
Acta Chimica Slovaca
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
2
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| Issue number |
2
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| ISSN |
1337-978X
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| Start page |
129
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| End page |
138
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| Total pages |
10
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| Place of publication |
Bratislava
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| Publisher |
Slovak Technical University
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| Collection year |
2009
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| Language |
english
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| Subject |
250000 Chemical Sciences
250400 Analytical Chemistry
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| Abstract/Summary |
In this work we present an electrochemical DNA biosensor arranged in a flow-through cell which was used for the evaluation of DNA damage. The DNA-based biosensor is created using a layer double stranded calf thymus DNA deposited on the surface of a screen-printed carbon electrode. The DNA damage by the Fenton reaction agents is detected by cyclic voltammetry parameters of the [Fe(CN)6]3–/4- complex used as a redox indicator in the phosphate buffer solution. Electrochemical impedance spectroscopy is used for the verification of changes of the DNA layer. The biosensor was also used for the detection of antioxidative effects of standard chemicals and tea extracts.
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| Keyword(s) |
DNA damage
biosensor
flow-through electrochemical system
screen-printed carbon electrode
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