Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants

Šimková, Darina, Beinrohr, Ernest and Labuda, Ján Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants Acta Chimica Slovaca, Vol.2, No. 2, 2009, 129-138

Document type: Článok z časopisu / Journal Article
Collection: Acta Chimica Slovaca  
 
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Author(s) Šimková, Darina
Beinrohr, Ernest
Labuda, Ján
Title Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants
Journal name Acta Chimica Slovaca
Publication date 2009
Year available 2009
Volume number 2
Issue number 2
ISSN 1337-978X
Start page 129
End page 138
Total pages 10
Place of publication Bratislava
Publisher Slovak Technical University
Collection year 2009
Language english
Subject 250000 Chemical Sciences
250400 Analytical Chemistry
Abstract/Summary In this work we present an electrochemical DNA biosensor arranged in a flow-through cell which was used for the evaluation of DNA damage. The DNA-based biosensor is created using a layer double stranded calf thymus DNA deposited on the surface of a screen-printed carbon electrode. The DNA damage by the Fenton reaction agents is detected by cyclic voltammetry parameters of the [Fe(CN)6]3–/4- complex used as a redox indicator in the phosphate buffer solution. Electrochemical impedance spectroscopy is used for the verification of changes of the DNA layer. The biosensor was also used for the detection of antioxidative effects of standard chemicals and tea extracts.
Keyword(s) DNA damage
biosensor
flow-through electrochemical system
screen-printed carbon electrode
 
 
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