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Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies
Gramblička, Michal, Tóthová, Darina, Antošová, Monika and Polakovič, Milan Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies Acta Chimica Slovaca, Vol.1, No. 1, 2008, -
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
acs_0008.pdf
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acs_0008.pdf |
application/pdf |
197.66KB |
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| Author(s) |
Gramblička, Michal
Tóthová, Darina
Antošová, Monika
Polakovič, Milan
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| Title |
Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies
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| Journal name |
Acta Chimica Slovaca
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
1
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| Issue number |
1
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| ISBN |
978-80-227-2957-4
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| Place of publication |
Bratislava
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| Publisher |
Slovak Technical University
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| Collection year |
2008
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| Language |
english
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| Subject |
290000 Engineering and Technology
290600 Chemical Engineering
270000 Biological Sciences
270100 Biochemistry and Cell Biology
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| Abstract/Summary |
Static adsorption capacity was measured for three different proteins: human immunoglobulin gamma, human serum albumin and horse skeletal muscle myoglobin. Eight commercial chromatographic materials were tested: FractoGel EMD SE HiCap (M), SP Sepharose Fast Flow, S Ceramic HyperD F, MabSelect, rProtein A Sepharose Fast Flow, Poros 50A High Capacity, ProSep –vA High Capacity and MEP HyperCel. The adsorption capacity was measured at pH values of 4.0, 5.0, 6.0, 7.0 and 8.0.
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| Keyword(s) |
adsorption capacity
chromatography
horse myoglobin
human immunoglobulin gamma
human serum albumin
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