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Tests of lipid peroxidation products using the DNA based electrochemical biosensor
Ferancová, Adriana, Beníková, Katarína, Galandová, Júlia, Sirotová, Ľudmila and Labuda, Ján Tests of lipid peroxidation products using the DNA based electrochemical biosensor Acta Chimica Slovaca, Vol.1, No. 1, 2008, 58-71
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
acs_0006.pdf
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acs_0006.pdf |
application/pdf |
445.68KB |
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| Author(s) |
Ferancová, Adriana
Beníková, Katarína
Galandová, Júlia
Sirotová, Ľudmila
Labuda, Ján
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| Title |
Tests of lipid peroxidation products using the DNA based electrochemical biosensor
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| Journal name |
Acta Chimica Slovaca
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
1
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| Issue number |
1
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| ISBN |
978-80-227-2957-4
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| Start page |
58
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| End page |
71
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| Total pages |
14
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| Place of publication |
Bratislava
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| Publisher |
Slovak Technical University
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| Collection year |
2008
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| Language |
english
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| Subject |
250000 Chemical Sciences
250400 Analytical Chemistry
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| Abstract/Summary |
In this work screen-printed carbon electrodes (SPCE) were modified with different nanocomposites: chitosan - multi-walled carbon nanotubes (CHIT-MWNT/SPCE), chitosan - single-walled carbon nanotubes (CHIT-SWNT/SPCE) and chitosan - carboxylated single-walled carbon nanotubes (CHIT-SWNT-COOH/SPCE) and further modified with DNA. Cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize basic electrochemical properties of the prepared sensors and biosensors. For the lipid peroxidation tests, DNA was first incubated with malondialdehyde (MDA) and oxidized edible oils, then isolated and put onto the surface of CHIT-SWNT-COOH/SPCE. Adducts formation of MDA and lipid peroxidation products with DNA was found. These adducts inhibit the diffusion of the electrochemical probe [Fe(CN)6]3−/4− to the electrode surface and, thus, changes in the relative charge-tranfer resistance were observed. These changes are in good agreement with the amount of lipid peroxidation products determined by the peroxide and TBARS values.
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| Keyword(s) |
DNA biosensor
lipid peroxidation
screen-printed carbon electrode
chitosan
carbon nanotubes
edible oils
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