Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

Şenel, Mehmet, Coşkun, Agah, Abasıyanık, M. Fatih and Bozkurt, Ayhan Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network Chemical Papers, Vol.64, No. 1, 2010, 1-7

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  

Author(s) Şenel, Mehmet
Coşkun, Agah
Abasıyanık, M. Fatih
Bozkurt, Ayhan
Title Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network
Journal name Chemical Papers
Publication date 2010
Year available 2010
Volume number 64
Issue number 1
ISSN 0366-6352
Start page 1
End page 7
Place of publication Poland
Publisher Versita
Collection year 2010
Language english
Subject 250000 Chemical Sciences
250500 Macromolecular Chemistry
Abstract/Summary In this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.
 
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