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Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network
Şenel, Mehmet, Coşkun, Agah, Abasıyanık, M. Fatih and Bozkurt, Ayhan Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network Chemical Papers, Vol.64, No. 1, 2010, 1-7
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Şenel, Mehmet
Coşkun, Agah
Abasıyanık, M. Fatih
Bozkurt, Ayhan
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| Title |
Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network
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| Journal name |
Chemical Papers
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| Publication date |
2010
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| Year available |
2010
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| Volume number |
64
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| Issue number |
1
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| ISSN |
0366-6352
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| Start page |
1
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| End page |
7
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2010
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| Language |
english
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| Subject |
250000 Chemical Sciences
250500 Macromolecular Chemistry
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| Abstract/Summary |
In this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.
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