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Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
Vojtylová, Terézia, Dospivová, Dana, Třísková, Olga, Pilařová, Iveta, Lubal, Přemysl, Farková, Marta, Trnková, Libuše and Táborský, Petr Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Chemical Papers, Vol.63, No. 6, 2009, 731-737
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Vojtylová, Terézia
Dospivová, Dana
Třísková, Olga
Pilařová, Iveta
Lubal, Přemysl
Farková, Marta
Trnková, Libuše
Táborský, Petr
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| Title |
Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
6
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| ISSN |
0366-6352
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| Start page |
731
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| End page |
737
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
250000 Chemical Sciences
250300 Organic Chemistry
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| Abstract/Summary |
Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 ± 0.1) °C): adenine (pK HL = 9.65 ± 0.04, pK H2L = 4.18 ± 0.04), adenosine (pK H2L = 3.59 ± 0.05), cytosine (pK H2L = 4.56 ± 0.01), cytidine (pK H2L = 4.16 ± 0.02). In addition, thermodynamic parameters for bases: adenine (ΔH 0 = (−17 ± 4) kJ mol−1, ΔS 0 = (23 ± 13) J K−1 mol−1), cytosine (ΔH 0 = (−22 ± 1) kJ mol−1, ΔS 0 = (13 ± 5) J K−1 mol−1) were calculated. Acidobasic behavior of oligonucleotides (5′CAC-CAC-CAC3′ = (CAC)3, 5′AAA-CCC-CCC3′ = A3C6, 5′CCC-AAA-CCC3′ = C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C+-C) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature.
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