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Novel use of silicon nanocrystals and nanodiamonds in biology
Fucikova, Anna, Valenta, Jan, Pelant, Ivan and Brezina, Vitezslav Novel use of silicon nanocrystals and nanodiamonds in biology Chemical Papers, Vol.63, No. 6, 2009, 704-708
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Fucikova, Anna
Valenta, Jan
Pelant, Ivan
Brezina, Vitezslav
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| Title |
Novel use of silicon nanocrystals and nanodiamonds in biology
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
6
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| ISSN |
0366-6352
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| Start page |
704
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| End page |
708
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
250000 Chemical Sciences
250500 Macromolecular Chemistry
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| Abstract/Summary |
The presented work is aimed at the development of nontoxic nanocrystalline silicon fluorescence labels, biodegradable in living body and long-term stable, and of fluorescent nanodiamonds mainly for in vitro use. These novel fluorescence labels could be very good substitutes for commercially used quantum dots (e.g. cadmium compound quantum dots) which can be toxic according to the latest results. In this work, manufacturing of porous nanocrystalline silicon (por-Si) is described, several basic optical properties of por-Si are presented and the influence of Si nanocrystals, nanodiamonds, and milled silicon on the growth of a cell culture of L929 mouse fibroblast and HeLa cells is compared. Bio-interaction of nanoparticles was studied by optical transmission microscopy, time-lapse microphotography of cell culture evolution, fluorescence microscopy, fluorescence microspectroscopy, and scanning electron microscopy. The size and shape of nanocrystals were determined using atomic force microscopy (AFM).
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