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Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex-DNA interactions
Colak, Ahmet, Cekirge, Ender, Karaböcek, Serdar, Küçükdumlu, Aslıgül, Ertunga, Nagihan Sağlam, Col, Melek and Abbasoğlu, Rza Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex-DNA interactions Chemical Papers, Vol.63, No. 5, 2009, 554-561
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Colak, Ahmet
Cekirge, Ender
Karaböcek, Serdar
Küçükdumlu, Aslıgül
Ertunga, Nagihan Sağlam
Col, Melek
Abbasoğlu, Rza
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| Title |
Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex-DNA interactions
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
5
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| ISSN |
0366-6352
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| Start page |
554
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| End page |
561
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
250000 Chemical Sciences
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| Abstract/Summary |
Nucleolytic activities of some new oxime-type ligand complexes were investigated by neutral agarose gel electrophoresis. Analysis of the cleavage products in agarose gel indicated that all complexes used converted supercoiled pUC18 plasmid DNA to its nicked or linear form. It was found that nucleolytic activities of the complexes depend on the complex concentration, reaction time and the presence of a cooxidant (magnesium monoperoxyphthalate, MMPP) in the reaction mixture. However, the complexes cleaved pUC18 plasmid DNA at all investigated pH values. Nucleolytic activities of complexes were investigated for different complex concentrations (0.1–100 μmol L−1), pH values (6.0–10.0) and reaction times (0–60 min). Molecular modeling studies performed by the Hyperchem Software together with DNA-binding studies showed that planar sites of the complexes intercalated into double stranded DNA. It can be concluded that all oxime-type ligand complexes used can be evaluated as nuclease mimics.
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