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Characterization of recombinant antibodies for detection of TNT and its derivatives
Simonoviíc, Mladen, Milosević-Zlatanović, Svetlana, Milosavić, Nenad, Vrvić, Miroslav M. and Simonović, Branislav Characterization of recombinant antibodies for detection of TNT and its derivatives Chemical Papers, Vol.63, No. 4, 2009, 391-398
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Simonoviíc, Mladen
Milosević-Zlatanović, Svetlana
Milosavić, Nenad
Vrvić, Miroslav M.
Simonović, Branislav
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| Title |
Characterization of recombinant antibodies for detection of TNT and its derivatives
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| Journal name |
Chemical Papers
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| Publication date |
2009
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| Year available |
2009
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| Volume number |
63
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| Issue number |
4
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| ISSN |
0366-6352
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| Start page |
391
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| End page |
398
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2009
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| Language |
english
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| Subject |
250000 Chemical Sciences
250100 Physical Chemistry (incl. Structural)
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| Abstract/Summary |
Diverse recombinant immunoreagents specific for TNT-derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP-protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT-analog TNP-Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
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