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Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y
Feng, Suling and Liu, Xueping Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y Chemical Papers, Vol.62, No. 3, 2008, 318-322
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Feng, Suling
Liu, Xueping
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| Title |
Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y
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| Journal name |
Chemical Papers
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| Publication date |
2008
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| Year available |
2008
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| Volume number |
62
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| Issue number |
3
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| ISSN |
0366-6352
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| Start page |
318
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| End page |
322
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2008
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| Language |
english
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| Subject |
250000 Chemical Sciences
250300 Organic Chemistry
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| Abstract/Summary |
A novel kinetic spectrofluorimetric method for the determination of uric acid based on the activation effect of uric acid on the Cu(II) ion catalyzed oxidation of pyronine Y by hydrogen peroxide was developed. The influence of different buffer solutions was tested and the Britton-Robinson buffer solution with pH 2.2 was found to be the optimum. The detection limit and the linear range for uric acid are 0.09 μg mL−1 and 0.3–3.0 μg mL−1, respectively. The RSD for eleven determinations of 1.6 μg mL−1 uric acid was 1.6 %. Satisfactory results were obtained when using this method of uric acid determination in human urine.
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