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Synthesis and identification of immunogen medroxyprogesterone acetate residues in edible foods and preparation of the Antisera
Xu, C. L., Peng, C. F., Hao, K., Jin, Z. Y. and Yang, S. Q. Synthesis and identification of immunogen medroxyprogesterone acetate residues in edible foods and preparation of the Antisera Chemical Papers, Vol.60, No. 1, 2006, 61-64
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Author(s) |
Xu, C. L.
Peng, C. F.
Hao, K.
Jin, Z. Y.
Yang, S. Q.
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| Title |
Synthesis and identification of immunogen medroxyprogesterone acetate residues in edible foods and preparation of the Antisera
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| Journal name |
Chemical Papers
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| Publication date |
2006
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| Year available |
2006
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| Volume number |
60
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| Issue number |
1
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| ISSN |
0366-6352
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| Start page |
61
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| End page |
64
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2006
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| Language |
english
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| Subject |
250000 Chemical Sciences
250300 Organic Chemistry
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| Abstract/Summary |
Medroxyprogesterone acetate (MPA), relative molecular mass is only 344.5 and it has no immunogenecity. The methods of the carbodiimides and the mixed anhydride were both adopted to couple MPA with bovine serum albumin, the carrier protein. The coupling rates of conjugate using the above methods were estimated to be 14 and 20 by ultraviolet spectrophotometry. The coupling was successful according to the analysis of SDS-PAGE illustration. New Zealand white rabbits were immunized with the conjugate the coupling rate of which was 14, and blood was collected after five periods of immunities. Then the titre of antiserum was tested to be 2.6 × 105 by indirect ELISA, which further identified the success of coupling.
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