 |
Stereoselective Reduction of 2-Phenylpropionaldehyde by Alcohol Dehydrogenase with Cofactor Regeneration
Kelemen-Horváth, I., Nemestóthy, N., Bélafi-Bakó, K. and Gubicza, L. Stereoselective Reduction of 2-Phenylpropionaldehyde by Alcohol Dehydrogenase with Cofactor Regeneration Chemical papers, Vol.56, No. 1, 2002, 52-56
|
|
|
Document type:
|
Článok z časopisu / Journal Article |
|
Collection:
|
Chemical papers
|
| |
| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
n561a52.pdf
|
561a52.pdf |
application/pdf |
169.22KB |
0 |
| Author(s) |
Kelemen-Horváth, I.
Nemestóthy, N.
Bélafi-Bakó, K.
Gubicza, L.
|
| Title |
Stereoselective Reduction of 2-Phenylpropionaldehyde by Alcohol Dehydrogenase with Cofactor Regeneration
|
| Journal name |
Chemical papers
|
| Publication date |
2002
|
| Year available |
2002
|
| Volume number |
56
|
| Issue number |
1
|
| ISSN |
0366-6352
|
| Start page |
52
|
| End page |
56
|
| Place of publication |
Poland
|
| Publisher |
Versita
|
| Collection year |
2002
|
| Language |
english
|
| Subject |
290000 Engineering and Technology
290600 Chemical Engineering
|
| Abstract/Summary |
Stereoselective reduction of 2-phenylpropionaldehyde catalyzed by alcoholdehydrogenase was investigated. Because of the water insolubility of thesubstrate and the target product (2-phenyl-1- propanol) organic solvent wasused. The process was intensified by the cofactor (NADH) regeneration in thesame media, using ethanol/acetaldehyde reaction as hydrogen carrier system. Theaim of the study was to realize the enzymatic process and in situ cofactorregeneration, simultaneously. Laboratory experiments in shake flasks system were carried out todetermine the optimal reaction conditions. Gas chromatography was used tofollow the conversion and the enantioselectivity of this reaction. Differentorganic solvents were tested and isopropyl ether was found the most suitable. Inwater–isopropyl ether solvent system (φr(VW/VO) = 37/63), optically pure (min. 95 %) (S)-2- phenyl-1-propanol was producedwith 25% yield after 2 h of reaction time.
|
|
|
|
