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Theoretical Study of Discrimination of Mechanisms of Thermal Inactivation of Urease by Monitoring its Oligomeric Composition
Illeová, V. and Polakovič, M. Theoretical Study of Discrimination of Mechanisms of Thermal Inactivation of Urease by Monitoring its Oligomeric Composition Chemical Papers, Vol.58, No. 1, 2004, 64-70
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
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n581a64.pdf
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581a64.pdf |
application/pdf |
189.37KB |
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| Author(s) |
Illeová, V.
Polakovič, M.
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| Title |
Theoretical Study of Discrimination of Mechanisms of Thermal Inactivation of Urease by Monitoring its Oligomeric Composition
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| Journal name |
Chemical Papers
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| Publication date |
2004
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| Year available |
2004
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| Volume number |
58
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| Issue number |
1
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| ISSN |
0366-6352
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| Start page |
64
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| End page |
70
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2004
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| Language |
english
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| Subject |
290000 Engineering and Technology
290600 Chemical Engineering
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| Abstract/Summary |
The possibility to discriminate the three-step mechanisms of thermal inactivation of jack bean urease was analyzed, the models being suggested in a previous study from the activity data. The simulations of the concentration courses of individual structural forms of urease incorporated in the mechanisms were performed. The results showed that all mechanisms could be discriminated if the dynamics of the evolution of oligomeric structural forms of urease was monitored. A careful choice of temperatures and combination of several analytical techniques is, however, required to achieve a reliable discrimination of the inactivation mechanism.
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