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Enhancementof the Thermostability of Candida cylindracea Lipase by Medium Engineering
Gubicza, L., Székely, K., Ulbert, O. and Bélafi-Bakó, K. Enhancementof the Thermostability of Candida cylindracea Lipase by Medium Engineering Chemical Papers, Vol.54, No. 6a, 2000, 351-354
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Document type:
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Článok z časopisu / Journal Article |
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Collection:
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Chemical papers
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| Attached Files |
| Name |
Description |
MIMEType |
Size |
Downloads |
n546aa351.pdf
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546aa351.pdf |
application/pdf |
151.82KB |
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| Author(s) |
Gubicza, L.
Székely, K.
Ulbert, O.
Bélafi-Bakó, K.
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| Title |
Enhancementof the Thermostability of Candida cylindracea Lipase by Medium Engineering
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| Journal name |
Chemical Papers
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| Publication date |
2000
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| Year available |
2000
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| Volume number |
54
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| Issue number |
6a
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| ISSN |
0366-6352
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| Start page |
351
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| End page |
354
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| Place of publication |
Poland
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| Publisher |
Versita
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| Collection year |
2000
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| Language |
english
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| Subject |
290000 Engineering and Technology
290600 Chemical Engineering
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| Abstract/Summary |
The possibility to increase the thermostability of the enzyme Candida cylindracea lipase was studied using the medium engineering method. The purpose was to shift the property of the enzyme obtained from mesophilic organism towards thermophilic character and hence widen its application possibilities. Applying the heptane solvent as reaction medium, the relation between the water content of the reaction mixture and the enzyme activity was investigated in the temperature range of 30ºC and 50ºC. It was found that decreasing the water content of the reaction mixture, the activity of the enzyme was reduced, while its thermostability increased. The slower deactivation of the enzyme was explained by the reduced amount of water present, necessary for the denaturation reactions.
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