Thermal Stability of Fructosyltransferase from Aureobasidium pullulans

Madlová, A., Antošová, M., Polakovič, M. and Báleš, V. Thermal Stability of Fructosyltransferase from Aureobasidium pullulans Chemical Papers, Vol.54, No. 6a, 2000, 339-344

Document type: Článok z časopisu / Journal Article
Collection: Chemical papers  
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Author(s) Madlová, A.
Antošová, M.
Polakovič, M.
Báleš, V.
Title Thermal Stability of Fructosyltransferase from Aureobasidium pullulans
Journal name Chemical Papers
Publication date 2000
Year available 2000
Volume number 54
Issue number 6a
ISSN 0366-6352
Start page 339
End page 344
Place of publication Poland
Publisher Versita
Collection year 2000
Language english
Subject 290000 Engineering and Technology
290600 Chemical Engineering
Abstract/Summary The thermal stability of a biocatalyst in the form of whole cells of the fungi Aureobasidium pullulans exhibiting fructosyltransferase activity was investigated during a batch catalytic process of fructooligosaccharide production. The stability of fructosyltransferase was monitored during 8 h with respect to temperature and overall saccharide concentration. A fructosyltransferase activity assay was developed for the monitoring of enzyme activity that was based on the rate of formation of 1-kestose measured by an HPLC method. It was found that the biocatalyst inactivated rather quickly at temperatures above 60ºC even at high saccharide concentrations. The stabilizing effect of saccharide concentration on the fructosyltransferase activity was investigated at 55ºC. A kinetic equation of enzyme inactivation, based on a reversible one-step reaction and simple exponential relationships of the rate constants and on the overall saccharide concentration, was suggested and statistically verified.
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